Cloning and Expression of a Novel Leucine Dehydrogenase: Characterization and L-tert-Leucine Production
نویسندگان
چکیده
منابع مشابه
Enzymatic synthesis of L-tert-leucine with branched chain aminotransferase.
In this study, we demonstrated the asymmetric synthesis of L-tert-leucine from trimethylpyruvate using branchedchain aminotransferase (BCAT) from Escherichia coli in the presence of L-glutamate as an amino donor. Since BCAT was severely inhibited by 2-ketoglutarate, in order to overcome this here, we developed a BCAT/aspartate aminotransferase (AspAT) and BCAT/AspAT/pyruvate decarboxylase (PDC)...
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ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2020
ISSN: 2296-4185
DOI: 10.3389/fbioe.2020.00186